LOCUS       40924_49187        3493 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Broad host range vector vector pCVD035, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3493)
  AUTHORS   Taton A, Unglaub F, Wright NE, Zeng WY, Paz-Yepez J, Brahamsha B, 
            Palenik B, Peterson TC, Haerizadeh F, Golden SS, Golden JW.
  TITLE     Broad-host-range vector system for synthetic biology and 
            biotechnology in cyanobacteria
  JOURNAL   Nucleic Acids Res. (2014) In press
  PUBMED    25074377
REFERENCE   2  (bases 1 to 3493)
  AUTHORS   Taton A, Unglaub F, Wright N, Zeng WY, Paz Yepes J, Brahamsha B, 
            Palenik B, Peterson T, Haerizadeh F, Golden SS, Golden JW.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JUN-2014) Division of Biological Sciences, University 
            of California, San Diego, 9500 Gilman Dr., La Jolla, CA 92093, USA
REFERENCE   3  (bases 1 to 3493)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3493)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. (2014) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (17-JUN-2014) Division of Biological Sciences, University of 
            California, San Diego, 9500 Gilman Dr., La Jolla, CA 92093, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3493
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     293..309
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        320..338
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    389..394
                     /label=EcoRV
                     /note="EcoRV"
     misc_feature    392..412
                     /note="C2G; GC-adaptor for seamless assembly, flanking 
                     antibiotic resistance markers, functional devices or custom
                     sequences; synthetic"
     misc_feature    413..418
                     /label=AgeI
                     /note="AgeI"
     regulatory      428..618
                     /note="PpsbAI_S7942; upstream region of psbA1 up to 25nt 
                     upstream of the start codon in Synechococcus elongatus 
                     PCC7942"
                     /regulatory_class="promoter"
     regulatory      625..665
                     /note="RBS_LT; RBS and flanking sequences found in Life
                     Tech pENTR-SD/D Topo, start downstream of the att site and 
                     includes the T7 gene enhancer, RBS, TOPO binding site and 
                     directional TOPO overhang; Life Technologies; synthetic"
                     /regulatory_class="ribosome_binding_site"
     RBS             636..656
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             666..1382
                     /label=EYFP
                     /note="enhanced YFP"
     protein_bind    complement(1402..1426)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      1436..1463
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     misc_feature    1472..1477
                     /label=XbaI
                     /note="XbaI"
     misc_feature    1478..1498
                     /note="G5C5; GC-adaptor for seamless assembly, flanking 
                     functional devices, custom sequences or cyanobacterial 
                     replicons; synthetic"
     misc_feature    1496..1501
                     /label=EcoRV
                     /note="EcoRV"
     rep_origin      complement(1746..2334)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2508..3365)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3366..3470)
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtggccgct acagggcgct cccattcgcc attcaggctg cgcaactgtt
      181 gggaagggcg tttcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
      241 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
      301 acggccagtg agcgcgacgt aatacgactc actatagggc gaattggcgg aaggccgtca
      361 aggccacgtg tcttgtccag gcgcgccaga tatcccgccg ccgccgccgg ataccggttt
      421 cgaattggga tttagcgtct tctaatccag tgtagacagt agttttggct ccgttgagca
      481 ctgtagcctt gggcgatcgc tctaaacatt acataaattc acaaagtttt cgttacataa
      541 aaatagtgtc tacttagcta aaaattaagg gttttttaca cctttttgac agttaatctc
      601 ctagcctaaa aagcaagaag atctccgcgg ccgccttgtt taactttaag aaggagccct
      661 tcaccatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc
      721 tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca
      781 cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc
      841 ccaccctcgt gaccaccttc ggctacggcc tgcagtgctt cgcccgctac cccgaccaca
      901 tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca
      961 tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaggttc gagggcgaca
     1021 ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg
     1081 ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga
     1141 agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc
     1201 tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca
     1261 accactacct gagctaccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca
     1321 tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca
     1381 agtaaaaggg tgggcgcgcc gacccagctt tcttgtacaa actcggccct gcaggagaag
     1441 gccatcctga cggatggcct ttttgaagct ttctagaatc gggggccccc ggggggatat
     1501 cattaattaa tggagcacaa gactggcctc atgggccttc cgctcactgc ccgctttcca
     1561 gtcgggaaac ctgtcgtgcc agctgcatta acatggtcat agctgtttcc ttgcgtattg
     1621 ggcgctctcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggg taaagcctgg
     1681 ggtgcctaat gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     1741 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     1801 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     1861 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     1921 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     1981 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     2041 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     2101 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     2161 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     2221 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     2281 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     2341 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     2401 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     2461 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     2521 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     2581 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     2641 ccgcgagaac cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     2701 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     2761 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     2821 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     2881 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     2941 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     3001 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     3061 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     3121 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     3181 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     3241 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     3301 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     3361 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     3421 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     3481 cgaaaagtgc cac
//
